Highly Sensitive Detection of Clenbuterol in Animal Urine Using Immunomagnetic Bead Treatment and Surface-Enhanced Raman Spectroscopy.

ABSTRACT: Combining surface-enhanced Raman spectroscopy (SERS) of aggregated graphene oxide/gold nanoparticle hybrids with immunomagnetic bead sample preparation method, a highly sensitive strategy to determine the clenbuterol content in animal urine was developed. Based on a linear calibration curve of the SERS characteristic peak intensity of clenbuterol at Δv = 1474 cm(-1) versus the spiked clenbuterol concentration in the range of 0.5-20 ng·mL(-1), the quantity of clenbuterol in real animal urine samples can be determined and matches well with those determined by LC-MS/MS, while the detection time is significantly reduced to 15 min/sample. The limits of detection and quantification in the urine are 0.5 ng·mL(-1) and 1 ng·mL(-1), respectively, and the recovery clenbuterol rates are 82.8-92.4% wit

SUBMITTER: Cheng J 

PROVIDER: S-EPMC5013518 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature