P66Shc, a key regulator of metabolism and mitochondrial ROS production, is dysregulated by mouse embryo culture.

ABSTRACT:

Study question

Do high oxygen tension and high glucose concentrations dysregulate p66Shc (Src homologous-collagen homologue adaptor protein) expression during mouse preimplantation embryo culture?

Summary answer

Compared with mouse blastocysts in vivo, P66Shc mRNA and protein levels in blastocysts maintained in vitro increased under high oxygen tension (21%), but not high glucose concentration.

What is known already

Growth in culture adversely impacts preimplantation embryo development and alters the expression levels of the oxidative stress adaptor protein p66Shc, but it is not known if p66Shc expression is linked to metabolic changes observed in cultured embryos.

Study design, samples/materials, methods

We used a standard wild-type CD1 mouse model of preimpla

SUBMITTER: Edwards NA 

PROVIDER: S-EPMC5013870 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature