In vitro differentiation of rat spermatogonia into round spermatids in tissue culture.

ABSTRACT:

Study question

Do the organ culture conditions, previously defined for in vitro murine male germ cell differentiation, also result in differentiation of rat spermatogonia into post-meiotic germ cells exhibiting specific markers for haploid germ cells?

Summary answer

We demonstrated the differentiation of rat spermatogonia into post-meiotic cells in vitro, with emphasis on exhibiting, protein markers described for round spermatids.

What is known already

Full spermatogenesis in vitro from immature germ cells using an organ culture technique in mice was first reported 5 years ago. However, no studies reporting the differentiation of rat spermatogonia into post-meiotic germ cells exhibiting the characteristic protein expression profile or into functional sperm have been r

SUBMITTER: Reda A 

PROVIDER: S-EPMC5013872 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature