HSD1 and AQP7 short-term gene regulation by cortisone in 3T3-L1 adipocytes.

ABSTRACT: Adipose Tissue (AT) is a complex organ with a crucial regulatory role in energy metabolism and in the development of obesity and the Metabolic Syndrome (MS). Modified responses and the metabolism of hormones have been observed in visceral adiposity during obesity, specifically as related with cortisone. The objective of this study was to assess, in the 3T3-L1 adipocyte cell line, the short-term effect of cortisone on the expression of 11β-Hydroxysteroid dehydrogenase 1 (Hsd1), which is responsible for activation of cortisone into cortisol, and for Aquaporin 7 (Aqp7), involved in glycerol transport through the cell membrane. Total RNA (tRNA) and complementary DNA (cDNA) were obtained from cell samples treated with cortisone (0.1, 1, and 10 μM) during different times (0, 5, 10, 15, and 20 mi

SUBMITTER: Quesada-Lopez T 

PROVIDER: S-EPMC5014000 | biostudies-literature | 2016 Jul-Sep

REPOSITORIES: biostudies-literature