Histone deacetylase inhibition impairs normal intestinal cell proliferation and promotes specific gene expression.

ABSTRACT: Mechanisms that maintain proliferation and delay cell differentiation in the intestinal crypt are not yet fully understood. We have previously shown the implication of histone methylation in the regulation of enterocytic differentiation. In this study, we investigated the role of histone deacetylation as an important epigenetic mechanism that controls proliferation and differentiation of intestinal cells using the histone deacetylase inhibitor suberanilohydroxamic acid (SAHA) on the proliferation and differentiation of human and mouse intestinal cells. Treatment of newly confluent Caco-2/15 cells with SAHA resulted in growth arrest, increased histone acetylation and up-regulation of the expression of intestine-specific genes such as those encoding sucrase-isomaltase, villin and the ion exc

SUBMITTER: Roostaee A 

PROVIDER: S-EPMC5014201 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature