Rhamnose Links Moonlighting Proteins to Membrane Phospholipid in Mycoplasmas.

ABSTRACT: Many proteins that have a primary function as a cytoplasmic protein are known to have the ability to moonlight on the surface of nearly all organisms. An example is the glycolytic enzyme enolase, which can be found on the surface of many types of cells from bacteria to human. Surface enolase is not enzymatic because it is monomeric and oligomerization is required for glycolytic activity. It can bind various molecules and activate plasminogen. Enolase lacks a signal peptide and the mechanism by which it attaches to the surface is unknown. We found that treatment of whole cells of the murine pathogen Mycoplasma pulmonis with phospholipase D released enolase and other common moonlighting proteins. Glycostaining suggested that the released proteins were glycosylated. Cytoplasmic and membrane-b

SUBMITTER: Daubenspeck JM 

PROVIDER: S-EPMC5014317 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature