Stabilization of Transfected Cells Expressing Low-Incidence Blood Group Antigens: Novel Methods Facilitating Their Use as Reagent-Cells.

ABSTRACT:

Background

The identification of erythrocyte antibodies in the serum of patients rely on panels of human red blood cells (RBCs), which coexpress many antigens and are not easily available for low-incidence blood group phenotypes. These problems have been addressed by generating cell lines expressing unique blood group antigens, which may be used as an alternative to human RBCs. However, the use of cell lines implies several drawbacks, like the requirement of cell culture facilities and the high cost of cryopreservation. The application of cell stabilization methods could facilitate their use as reagent cells in clinical laboratories.

Methods

We generated stably-transfected cells expressing low-incidence blood group antigens (Dia and Lua). High-expresser clones were used to a

SUBMITTER: Gonzalez C 

PROVIDER: S-EPMC5014343 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature