Lipid-based Transfection Reagents Exhibit Cryo-induced Increase in Transfection Efficiency.

ABSTRACT: The advantages of lipid-based transfection reagents have permitted their widespread use in molecular biology and gene therapy. This study outlines the effect of cryo-manipulation of a cationic lipid-based formulation, Lipofectamine 2000, which, after being frozen and thawed, showed orders of magnitude higher plasmid delivery efficiency throughout eight different cell lines, without compromising cell viability. Increased transfection efficiency with the freeze-thawed reagent was also seen with 2'-O-methyl phosphorothioate oligonucleotide delivery and in a splice-correction assay. Most importantly, a log-scale improvement in gene delivery using the freeze-thawed reagent was seen in vivo. Using three different methods, we detected considerable differences in the polydispersity of the differen

SUBMITTER: Sork H 

PROVIDER: S-EPMC5014464 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature