An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation.

ABSTRACT: Although rats are preferred over mice as an animal model, transgenic animals are generated predominantly using mouse embryos. There are limitations in the generation of transgenic rat by embryo manipulation. Unlike mouse embryos, most of the rat embryos do not survive after male pronuclear DNA injection which reduces the efficiency of generation of transgenic rat by this method. More importantly, this method requires hundreds of eggs collected by killing several females for insertion of transgene to generate transgenic rat. To this end, we developed a noninvasive and deathless technique for generation of transgenic rats by integrating transgene into the genome of the spermatogonial cells by testicular injection of DNA followed by electroporation. After standardization of this technique usi

SUBMITTER: Pradhan BS 

PROVIDER: S-EPMC5014465 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature