TGF-β1-induced PAI-1 contributes to a profibrotic network in patients with eosinophilic esophagitis.

ABSTRACT:

Background

Eosinophilic esophagitis (EoE) is an allergic disease of increasing worldwide incidence. Complications are due to tissue remodeling and involve TGF-β1-mediated fibrosis. Plasminogen activator inhibitor 1 (PAI-1/serpinE1) can be induced by TGF-β1, but its role in EoE is not known.

Objective

We sought to understand the expression and role of PAI-1 in patients with EoE.

Methods

We used esophageal biopsy specimens and plasma samples from control subjects and patients with EoE, primary human esophageal epithelial cells, and fibroblasts from patients with EoE in immunohistochemistry, quantitative PCR, and immunoassay experiments to understand the induction of PAI-1 by TGF-β1, the relationship between PAI-1 and esophageal fibrosis, and the role of PAI-1 in fibroti

SUBMITTER: Rawson R 

PROVIDER: S-EPMC5014565 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature