Clonal expansion of CD4(+) cytotoxic T lymphocytes in patients with IgG4-related disease.

ABSTRACT:

Background

IgG4-related disease (IgG4-RD) is a systemic condition of unknown cause characterized by highly fibrotic lesions with dense lymphoplasmacytic infiltrates. CD4(+) T cells constitute the major inflammatory cell population in IgG4-RD lesions.

Objective

We used an unbiased approach to characterize CD4(+) T-cell subsets in patients with IgG4-RD based on their clonal expansion and ability to infiltrate affected tissue sites.

Methods

We used flow cytometry to identify CD4(+) effector/memory T cells in a cohort of 101 patients with IgG4-RD. These expanded cells were characterized by means of gene expression analysis and flow cytometry. Next-generation sequencing of the T-cell receptor β chain gene was performed on CD4(+)SLAMF7(+) cytotoxic T lymphocytes (CTLs) and

SUBMITTER: Mattoo H 

PROVIDER: S-EPMC5014627 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature