Direct Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones.

ABSTRACT: Quantifying drug permeability across lipid membranes is crucial for drug development. In addition, reduced membrane permeability is a leading cause of antibiotic resistance in bacteria, and hence there is a need for new technologies that can quantify antibiotic transport across biological membranes. We recently developed an optofluidic assay that directly determines the permeability coefficient of autofluorescent drug molecules across lipid membranes. Using ultraviolet fluorescence microscopy, we directly track drug accumulation in giant lipid vesicles as they traverse a microfluidic device while exposed to the drug. Importantly, our measurement does not require the knowledge of the octanol partition coefficient of the drug - we directly determine the permeability coefficient for the speci

SUBMITTER: Cama J 

PROVIDER: S-EPMC5015079 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature