Bacteriophage tailspike protein based assay to monitor phase variable glucosylations in Salmonella O-antigens.

ABSTRACT:

Background

Non-typhoid Salmonella Typhimurium (S. Typhimurium) accounts for a high number of registered salmonellosis cases, and O-serotyping is one important tool for monitoring epidemiology and spread of the disease. Moreover, variations in glucosylated O-antigens are related to immunogenicity and spread in the host. However, classical autoagglutination tests combined with the analysis of specific genetic markers cannot always reliably register phase variable glucose modifications expressed on Salmonella O-antigens and additional tools to monitor O-antigen glucosylation phenotypes of S. Typhimurium would be desirable.

Results

We developed a test for the phase variable O-antigen glucosylation state of S. Typhimurium using the tailspike proteins (TSP) of Salmonella phages 9N

SUBMITTER: Schmidt A 

PROVIDER: S-EPMC5015238 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature