Characterization of a pathway-specific activator of milbemycin biosynthesis and improved milbemycin production by its overexpression in Streptomyces bingchenggensis.

ABSTRACT:

Background

Milbemycins, a group of 16-membered macrolides with potent anthelminthic and insecticidal activity, are produced by several Streptomyces and used widely in agricultural, medical and veterinary fields. Milbemycin A3 and A4, the main components produced by Streptomyces bingchenggensis, have been developed as an acaricide to control mites. The subsequent structural modification of milbemycin A3/A4 led to other commercial products, such as milbemycin oxime, lepimectin and latidectin. Despite its importance, little is known about the regulation of milbemycin biosynthesis, which has hampered efforts to enhance milbemycin production via engineering regulatory genes.

Results

milR, a regulatory gene in the milbemycin (mil) biosynthetic gene cluster of S. bingchenggensis, e

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5015266 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature