Alternation of apoptotic and implanting genes expression of mouse embryos after re-vitrification.

ABSTRACT:

Background

Nowadays, oocytes and embryos vitrification has become a routine technique. Based on clinical judgment, re-vitrification maybe required. But little is known about re-vitrification impact on genes expression.

Objective

The impact of re-vitrification on apoptotic and implanting genes, Bax, Bcl-2 and ErbB4, at compaction stage embryos were evaluated in this study.

Materials and methods

In this experimental study, 8 cell embryos (n=240) were collected from female mature mice, 60-62 hr post HCG injection. The embryos were divided randomly to 3 groups included: fresh (n=80), vitrified at 8 cell stage (n=80), vitrified at 8 cell stage thawed and re-vitrified at compaction stage (n=80). Embryos were vitrified by using cryolock, (open system) described by Kuwayama.

SUBMITTER: Majidi Gharenaz N 

PROVIDER: S-EPMC5015665 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature