Development of a multifunctional aminoxy-based fluorescent linker for glycan immobilization and analysis.

ABSTRACT: Glycan arrays have become a technique of choice to screen glycan-protein interactions in a high-throughput manner with high sensitivity and low sample consumption. Here, the synthesis of a new multifunctional fluorescent linker for glycan labeling via aminoxy ligation and immobilization is described; the linker features a fluorescent naphthalene group suitable for highly sensitive high-performance liquid chromatography-based purification and an azido- or amino-modified pentanoyl moiety for the immobilization onto solid supports. Several glycoconjugates displaying small sugar epitopes via chemical or chemoenzymatic synthesis were covalently attached onto a microarray support and tested with lectins of known carbohydrate binding specificity. The glycan library was extended using glycosyltran

SUBMITTER: Jimenez-Castells C 

PROVIDER: S-EPMC5015709 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature