Sensitivity of Allelic Divergence to Genomic Position: Lessons from the Drosophila tan Gene.

ABSTRACT: To identify genetic variants underlying changes in phenotypes within and between species, researchers often utilize transgenic animals to compare the function of alleles in different genetic backgrounds. In Drosophila, targeted integration mediated by the ΦC31 integrase allows activity of alternative alleles to be compared at the same genomic location. By using the same insertion site for each transgene, position effects are generally assumed to be controlled for because both alleles are surrounded by the same genomic context. Here, we test this assumption by comparing the activity of tan alleles from two Drosophila species, D. americana and D. novamexicana, at five different genomic locations in D. melanogaster We found that the relative effects of these alleles varied among insertion sit

SUBMITTER: John AV 

PROVIDER: S-EPMC5015952 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature