The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins.

ABSTRACT: Fimbrins/plastins have been implicated in the generation of distinct actin structures, which are linked to different cellular processes. Historically, fimbrins/plastins were mainly considered as generating tight actin bundles. Here, we demonstrate that different members of the fimbrin/plastin family have diverged biochemically during evolution to generate either tight actin bundles or loose networks with distinct biochemical and biophysical properties. Using the phylogenetically and functionally distinct Arabidopsis fimbrins FIM4 and FIM5 we found that FIM4 generates both actin bundles and cross-linked actin filaments, whereas FIM5 only generates actin bundles. The distinct functions of FIM4 and FIM5 are clearly observed at single-filament resolution. Domain swapping experiments showed tha

SUBMITTER: Zhang R 

PROVIDER: S-EPMC5016177 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature