Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes.

ABSTRACT: Cold-active enzymes constitute an attractive resource for biotechnological applications. Their high catalytic activity at temperatures below 25°C makes them excellent biocatalysts that eliminate the need of heating processes hampering the quality, sustainability, and cost-effectiveness of industrial production. Here we provide a review of the isolation and characterization of novel cold-active enzymes from microorganisms inhabiting different environments, including a revision of the latest techniques that have been used for accomplishing these paramount tasks. We address the progress made in the overexpression and purification of cold-adapted enzymes, the evolutionary and molecular basis of their high activity at low temperatures and the experimental and computational techniques used for t

SUBMITTER: Santiago M 

PROVIDER: S-EPMC5016527 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature