Genetic toxicity assessment of engineered nanoparticles using a 3D in vitro skin model (EpiDerm™).

ABSTRACT:

Background

The rapid production and incorporation of engineered nanomaterials into consumer products alongside research suggesting nanomaterials can cause cell death and DNA damage (genotoxicity) makes in vitro assays desirable for nanosafety screening. However, conflicting outcomes are often observed when in vitro and in vivo study results are compared, suggesting more physiologically representative in vitro models are required to minimise reliance on animal testing.

Method

BASF Levasil® silica nanoparticles (16 and 85 nm) were used to adapt the 3D reconstructed skin micronucleus (RSMN) assay for nanomaterials administered topically or into the growth medium. 3D dose-responses were compared to a 2D micronucleus assay using monocultured human B cells (TK6) after standardisin

SUBMITTER: Wills JW 

PROVIDER: S-EPMC5016964 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature