Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies.

ABSTRACT: HGF-Met signaling contributes to various biological events by controlling cell migration. Since the abnormal activation of Met receptor causes cancer progression, inhibitors such as neutralizing antibodies are regarded as promising therapeutics. HGF is secreted as a single-chain (sc) precursor and is processed by extracellular proteases to generate disulfide-bonded two-chain (tc) HGF. Although this proteolytic processing of HGF is necessary for its biological activity, exactly how the proteolysis leads to the conversion of HGF to the active form is still unclar due to the lack of structural information. In order to gain insights about this point, we generated 6 antibodies against HGF. All antibodies recognized different epitopes on the native HGF protein and showed distinct effects when te

SUBMITTER: Umitsu M 

PROVIDER: S-EPMC5017023 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature