Iridoid glycoside permethylation enhances chromatographic separation and chemical ionization.

ABSTRACT:

Rationale

While natural products isolated from medicinal plants can serve as a rich source of biologically active metabolites, mixtures of structurally related compounds of a polar nature are often difficult to chemically resolve by traditional separation techniques. Chemical derivatization to reduce metabolite polarity combined with liquid chromatography (LC) is the strategy presented here to resolve a mixture of structurally related natural product glycosides solvent extracted from the medicinal herb Teucrium polium for mass spectrometric characterization.

Methods

The partially purified plant extract was subjected to chemical derivatization and electrospray ionization mass spectrometry (ESI-MS) fragmentation pattern analysis allowed for structural characterization of irido

SUBMITTER: Elmasri WA 

PROVIDER: S-EPMC5017310 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature