Optimization of Extraction Conditions for Maximal Phenolic, Flavonoid and Antioxidant Activity from Melaleuca bracteata Leaves Using the Response Surface Methodology.

ABSTRACT: Melaleuca bracteata is a yellow-leaved tree belonging to the Melaleuca genus. Species from this genus are known to be good sources of natural antioxidants, for example, the "tea tree oil" derived from M. alternifolia is used in food processing to extend the shelf life of products. In order to determine whether M. bracteata contains novel natural antioxidants, the components of M. bracteata ethanol extracts were analyzed by gas chromatography-mass spectrometry. Total phenolic and flavonoid contents were extracted and the antioxidant activities of the extracts evaluated. Single-factor experiments, central composite rotatable design (CCRD) and response surface methodology (RSM) were used to optimize the extraction conditions for total phenolic content (TPC) and total flavonoid content (TFC).

SUBMITTER: Hou W 

PROVIDER: S-EPMC5017642 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature