Impacts of the G145R Mutation on the Structure and Immunogenic Activity of the Hepatitis B Surface Antigen: A Computational Analysis.

ABSTRACT:

Background

Vaccine-escaped hepatitis B virus (HBV) mutations occur within the "a" determinant area, which is located in the major hydrophilic region (MHR) of the hepatitis B surface antigen (HBsAg) protein. It is now well established that the common G145R mutation is highly capable of escaping from HBsAg immune recognition. However, the impacts of this mutation on the structure and immunogenic activity of HBsAg have been poorly investigated.

Objectives

The present study analyzed the effects of the G145R mutation on the structure and immunogenic activity of the HBsAg.

Materials and methods

Three-dimensional (3D) structure of HBsAg for both the wild-type and G145R mutant were predicted and refined using several web tools. After quantitative evaluations, the effects of t

SUBMITTER: Rezaee R 

PROVIDER: S-EPMC5018363 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature