Developmental Expression of Ecdysone-Related Genes Associated With Metamorphic Changes During Midgut Remodeling of Silkworm Bombyx mori (Lepidoptera:Bombycidae).

ABSTRACT: Steroid hormone 20-hydroxyecdysone is known as the systemic regulators of insect cells; however, how to impact the fate and function of mature and stem cells is unclear. For the first time, we report ecdysone regulatory cascades in both mature midgut cell and stem cell fractions related to developmental events by using histological, immunohistochemical, biochemical and gene expression analysis methods. Ecdysone receptor-B1 (EcR-B1) and ultraspiracle 1 (USP-1) mRNAs were detected mainly in mature cells during programmed cell death (PCD). Lowered E75A and probably BR-C Z4 in mature cells appear to provide a signal to the initiation of PCD. E74B, E75B and BR-C Z2 seem to be early response genes which are involved in preparatory phase of cell death. It is likely that βFTZ-F1, E74A and BR-C Z1

SUBMITTER: Goncu E 

PROVIDER: S-EPMC5019025 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature