Genetic va riation in the na rrow-c lawed c ra yfish (Astacus lepto dactylus) po pulatio ns as assesse d by PCR-RFLP of mitochondria l COI gene.

ABSTRACT: The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PC R) restriction fragment length polymorphism (RF LP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each in Anzali wetland and Aras reservoir and three rivers Chafrood, Masule Rudkhan and S iah Darvishan. The PCR products were digested with 19 restriction enzymes and five enzymes revealed polymorphism patterns (DdeІ, MboІ, TaqI, RsaІ and HinfІ). Twenty eight composite haplotypes were showed with the number of haplotypes in each population sample rangin

SUBMITTER: Khos Hkholgh M 

PROVIDER: S-EPMC5019214 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature