Identification and characterization of a NBS-LRR class resistance gene analog in Pistacia atlantica subsp. Kurdica.

ABSTRACT: P. atlantica subsp. Kurdica, with the local name of Baneh, is a wild medicinal plant which grows in Kurdistan, Iran. The identification of resistance gene analogs holds great promise for the development of resistant cultivars. A PCR approach with degenerate primers designed according to conserved NBS-LRR (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (R) genes was used to amplify and clone homologous sequences from P. atlantica subsp. Kurdica. A DNA fragment of the expected 500-bp size was amplified. The nucleotide sequence of this amplicon was obtained through sequencing and the predicted amino acid sequence compared to the amino acid sequences of known R-genes revealed significant sequence similarity. Alignment of the deduced amino acid se

SUBMITTER: Bahramnejad B 

PROVIDER: S-EPMC5019225 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature