Single nucleotide polymorphism of FGF2 gene in Iranian Holstein proven bulls.

ABSTRACT: Fibroblast growth factor 2 (FGF2) serves in the uterine endometrium during estrous presenting in the bovine mammary gland which is responsible to express interferon-T (IFNT), and is an important agent to encourage the continuation of pregnancy in the ruminants. Significant associations have been found between genes affected by IFNT and genes that are responsible for milk production traits. Semen samples from 101Iranian Holstein proven bulls were collected to extract the genomic DNA. Forward and reverse primers were designed and a 710-base-pair fragment in intron 1 was amplified using PCR technique. To detect single nucleotide polymorphism (SNP), all samples were sequenced. Three positions including 11474 (C/G), 11513 (C/G) and 11646 (A/G) were considered. The 11474C, 1

SUBMITTER: Salehi A 

PROVIDER: S-EPMC5019300 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature