Identification of HIV Mutation as Diagnostic Biomarker through Next Generation Sequencing.

ABSTRACT:

Introduction

Current clinical detection of Human immunodeficiency virus 1 (HIV-1) is used to target viral genes and proteins. However, the immunoassay, such as viral culture or Polymerase Chain Reaction (PCR), lacks accuracy in the diagnosis, as these conventional assays rely on the stable genome and HIV-1 is a highly-mutated virus. Next generation sequencing (NGS) promises to be transformative for the practice of infectious disease, and the rapidly reducing cost and processing time mean that this will become a feasible technology in diagnostic and research laboratories in the near future. The technology offers the superior sensitivity to detect the pathogenic viruses, including unknown and unexpected strains.

Aim

To leverage the NGS technology in order to improve current HI

SUBMITTER: Shaw WH 

PROVIDER: S-EPMC5020253 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature