Development and evaluation of real-time loop-mediated isothermal amplification assay for rapid detection of cystic echinococcosis.

ABSTRACT:

Background

Cystic echinococcosis (CE) or hydatidosis, caused by the larval stage of Echinococcus granulosus (EG)-complex, is a neglected parasitic disease of public health importance. The disease is endemic in many African and Mediterranean countries including the Sudan. The objective of the present study was to develop and evaluate a real-time loop-mediated isothermal amplification (LAMP) assay for simple and rapid detection of CE in humans and domestic live stock in Sudan.

Methods

A set of six LAMP primers, designed from the mitochondrial NADH-1 gene of EG cattle strain of genotype 5 (G5), was used as a target for LAMP assay. The assay was performed at a constant temperature (63 °C), with a real-time follow-up using a LightCycler and fluorochrome dye. Following amplificati

SUBMITTER: Ahmed ME 

PROVIDER: S-EPMC5020552 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature