Reversion of Cold-Adapted Live Attenuated Influenza Vaccine into a Pathogenic Virus.

ABSTRACT:

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The only licensed live attenuated influenza A virus vaccines (LAIVs) in the United States (FluMist) are created using internal protein-coding gene segments from the cold-adapted temperature-sensitive master donor virus A/Ann Arbor/6/1960 and HA/NA gene segments from circulating viruses. During serial passage of A/Ann Arbor/6/1960 at low temperatures to select the desired attenuating phenotypes, multiple cold-adaptive mutations and temperature-sensitive mutations arose. A substantial amount of scientific and clinical evidence has proven that FluMist is safe and effective. Nevertheless, no study has been conducted specifically to determine if the attenuating temperature-sensitive phenotype can revert and, if so, the types of substitutions that will emerge (i.e., compensato

SUBMITTER: Zhou B 

PROVIDER: S-EPMC5021423 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature