Hook2, a microtubule-binding protein, interacts with Par6α and controls centrosome orientation during polarized cell migration.

ABSTRACT: Polarity protein complexes function during polarized cell migration and a subset of these proteins localizes to the reoriented centrosome during this process. Despite these observations, the mechanisms behind the recruitment of these polarity complexes such as the aPKC/PAR6α complex to the centrosome are not well understood. Here we identify Hook2 as an interactor for the aPKC/PAR6α complex that functions to localize this complex at the centrosome. We first demonstrate that Hook2 is essential for the polarized Golgi re-orientation towards the migration front. Depletion of Hook2 results in a decrease of PAR6α at the centrosome during cell migration, while overexpression of Hook2 in cells induced the formation of aggresomes with the recruitment of PAR6α, aPKC and PAR3. In addition, we demons

SUBMITTER: Pallesi-Pocachard E 

PROVIDER: S-EPMC5021942 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature