Contamination-controlled high-throughput whole genome sequencing for influenza A viruses using the MiSeq sequencer.

ABSTRACT: Accurate full-length genomic sequences are important for viral phylogenetic studies. We developed a targeted high-throughput whole genome sequencing (HT-WGS) method for influenza A viruses, which utilized an enzymatic cleavage-based approach, the Nextera XT DNA library preparation kit, for library preparation. The entire library preparation workflow was adapted for the Sentosa SX101, a liquid handling platform, to automate this labor-intensive step. As the enzymatic cleavage-based approach generates low coverage reads at both ends of the cleaved products, we corrected this loss of sequencing coverage at the termini by introducing modified primers during the targeted amplification step to generate full-length influenza A sequences with even coverage across the whole genome. Another challeng

SUBMITTER: Lee HK 

PROVIDER: S-EPMC5022032 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature