Synthesis of alpha-L-threofuranosyl nucleoside triphosphates (tNTPs).

ABSTRACT: [structure: see text] The alpha-l-threofuranosyl nucleoside triphosphates of T, G, and D (tTTP, tGTP, and tDTP) were synthesized from the described 2'-O-DMT-protected derivatives using the Eckstein method, while the corresponding C derivative (tCTP) was prepared from the 2'-O-acetyl derivative. The prepared alpha-l-threofuranosyl nucleoside triphosphates, despite being one carbon shorter than the native 2'-deoxyfuranosyl nucleoside triphosphates, are effective substrates for selected DNA polymerases.

SUBMITTER: Zou K 

PROVIDER: S-EPMC5022784 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature