Expression Profile of Developmentally Important Genes in preand peri-Implantation Goat Embryos Produced In Vitro.

ABSTRACT:

Background

Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and day 14 blastocyst).

Materials and methods

In this experimental study, stage-specific profiling using real time-quantitative polymerase chain reaction (RT-qPCR) revealed robust and dynamic patterns of stage-specific gene activity that fall into four major clusters depending on their respective mRNA profiles.

Results

The gradual pattern of reduction in the maternally stored transcripts without renewal thereafter (clust

SUBMITTER: HosseinNia P 

PROVIDER: S-EPMC5023042 | biostudies-literature | 2016 Oct-Dec

REPOSITORIES: biostudies-literature