Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands.

ABSTRACT: Peptides are important affinity ligands for microscopy, biosensing, and targeted delivery. However, because they can have low affinity for their targets, their selection from large naïve libraries can be challenging. When selecting peptidic ligands from display libraries, it is important to: 1) ensure efficient display; 2) maximize the ability to select high affinity ligands; and 3) minimize the effect of the display context on binding. The "helper cell" packaging system has been described as a tool to produce filamentous phage particles based on phagemid constructs with varying display levels, while remaining free of helper phage contamination. Here we report on the first use of this system for peptide display, including the systematic characterization and optimization of helper cells, th

SUBMITTER: Phipps ML 

PROVIDER: S-EPMC5023105 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature