Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis.

ABSTRACT:

Background

Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas.

Methodology/principal findings

Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for th

SUBMITTER: Gomes C 

PROVIDER: S-EPMC5023120 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature