Characterization of Intrinsic Properties of Promoters.

ABSTRACT: Accurate characterization of promoter behavior is essential for the rational design of functional synthetic transcription networks such as logic gates and oscillators. However, transcription rates observed from promoters can vary significantly depending on the growth rate of host cells and the experimental and genetic contexts of the measurement. Furthermore, in vivo measurement methods must accommodate variation in translation, protein folding, and maturation rates of reporter proteins, as well as metabolic load. The external factors affecting transcription activity may be considered to be extrinsic, and the goal of characterization should be to obtain quantitative measures of the intrinsic characteristics of promoters. We have developed a promoter characterization method that is based on

SUBMITTER: Rudge TJ 

PROVIDER: S-EPMC5023225 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature