Galaxy Workflows for Web-based Bioinformatics Analysis of Aptamer High-throughput Sequencing Data.

ABSTRACT: Development of RNA and DNA aptamers for diagnostic and therapeutic applications is a rapidly growing field. Aptamers are identified through iterative rounds of selection in a process termed SELEX (Systematic Evolution of Ligands by EXponential enrichment). High-throughput sequencing (HTS) revolutionized the modern SELEX process by identifying millions of aptamer sequences across multiple rounds of aptamer selection. However, these vast aptamer HTS datasets necessitated bioinformatics techniques. Herein, we describe a semiautomated approach to analyze aptamer HTS datasets using the Galaxy Project, a web-based open source collection of bioinformatics tools that were originally developed to analyze genome, exome, and transcriptome HTS data. Using a series of Workflows created in the Galaxy we

SUBMITTER: Thiel WH 

PROVIDER: S-EPMC5023399 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature