Effects of Fibrotic Tissue on Liver-targeted Hydrodynamic Gene Delivery.

ABSTRACT: Hydrodynamic gene delivery is a common method for gene transfer to the liver of small animals, and its clinical applicability in large animals has been demonstrated. Previous studies focused on functional analyses of therapeutic genes in animals with normal livers and little, however, is known regarding its effectiveness and safety in animals with liver fibrosis. Therefore, this study aimed to examine the effects of liver fibrosis on hydrodynamic gene delivery efficiency using a rat liver fibrosis model. We demonstrated for the first time, using pCMV-Luc plasmid, that this procedure is safe and that the amount of fibrotic tissue in the liver decreases gene delivery efficiency, resulting in decrease in luciferase activity depending on the volume of fibrotic tissue in the liver and the numbe

SUBMITTER: Kobayashi Y 

PROVIDER: S-EPMC5023407 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature