Bovine male germline stem-like cells cultured in serum- and feeder-free medium.

ABSTRACT: Male germline stem cells (mGSCs) presented in male testis are responsible for spermatogenesis during their whole life. However, little information can be found on the culture of bovine mGSCs, and the current culture system needs to be improved. In this study, we compared the effects of several commercial serum-free media and different extra-cellular matrix on the enrichment and cultivation of mGSCs. To find out the best culture condition, the biological characteristics of the cultured cells were evaluated by morphological observation, RT-PCR and immunofluorescent staining. According to the cells' condition in different experiment groups, we found out an efficient cultivation system for bovine mGSCs derived from neonate testis. In this serum- and feeder-free medium, the cultured cells maint

SUBMITTER: Li B 

PROVIDER: S-EPMC5023554 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature