Mapping centromeres of microchromosomes in the zebra finch (Taeniopygia guttata) using half-tetrad analysis.

ABSTRACT: Centromeres usually consist of hundreds of kilobases of repetitive sequence which renders them difficult to assemble. As a consequence, centromeres are often missing from assembled genomes and their locations on physical chromosome maps have to be inferred from flanking sequences via fluorescence in situ hybridization (FISH). Alternatively, centromere positions can be mapped using linkage analyses in accidentally triploid individuals formed by half-tetrads (resulting from the inheritance of two chromatids from a single meiosis). The current genome assembly of the zebra finch (Taeniopygia guttata) comprises 32 chromosomes, but only for the ten largest chromosomes centromere positions have been mapped using FISH. We here map the positions of most of the remaining centromeres using half-tetra

SUBMITTER: Knief U 

PROVIDER: S-EPMC5023761 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature