Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections.

ABSTRACT: The recent availability of novel dyes and alternative light sources to facilitate complex tissue immunofluorescence studies such as multiplex labelling has not been matched by reports critically evaluating the considerations and relative benefits of these new tools, particularly in combination. Product information is often limited to wavelengths used for older fluorophores (FITC, TRITC & corresponding Alexa dyes family). Consequently, novel agents such as Quantum dots are not widely appreciated or used, despite highly favourable properties including extremely bright emission, stability and potentially reduced tissue autofluorescence at the excitation wavelength. Using spectral analysis, we report here a detailed critical appraisal and comparative evaluation of different light sources and f

SUBMITTER: Prost S 

PROVIDER: S-EPMC5025086 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature