Multiparametric Functional MRI: Non-Invasive Imaging of Inflammation and Edema Formation after Kidney Transplantation in Mice.

ABSTRACT:

Background

Kidney transplantation (ktx) in mice is used to learn about rejection and to develop new treatment strategies. Past studies have mainly been based on histological or molecular biological methods. Imaging techniques to monitor allograft pathology have rarely been used.

Methods

Here we investigated mice after isogenic and allogenic ktx over time with functional MRI with diffusion-weighted imaging (DWI) and mapping of T2-relaxation time (T2-mapping) to assess graft inflammation and edema formation. To characterize graft pathology, we used PAS-staining, counted CD3-positive T-lymphocytes, analyzed leukocytes by means flow cytometry.

Results

DWI revealed progressive restriction of diffusion of water molecules in allogenic kidney grafts. This was paralleled by en

SUBMITTER: Hueper K 

PROVIDER: S-EPMC5025122 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature