Identification of extracellular miRNA in archived serum samples by next-generation sequencing from RNA extracted using multiple methods.

ABSTRACT: miRNAs act as important regulators of gene expression by promoting mRNA degradation or by attenuating protein translation. Since miRNAs are stably expressed in bodily fluids, there is growing interest in profiling these miRNAs, as it is minimally invasive and cost-effective as a diagnostic matrix. A technical hurdle in studying miRNA dynamics is the ability to reliably extract miRNA as small sample volumes and low RNA abundance create challenges for extraction and downstream applications. The purpose of this study was to develop a pipeline for the recovery of miRNA using small volumes of archived serum samples. The RNA was extracted employing several widely utilized RNA isolation kits/methods with and without addition of a carrier. The small RNA library preparation was carried out using Il

SUBMITTER: Gautam A 

PROVIDER: S-EPMC5025515 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature