In vivo detection of 2-hydroxyglutarate in brain tumors by optimized point-resolved spectroscopy (PRESS) at 7T.

ABSTRACT:

Purpose

To test the efficacy of 7T MRS for in vivo detection of 2-hydroxyglutarate (2HG) in brain tumors.

Methods

The subecho times of point-resolved spectroscopy (PRESS) were optimized at 7T with density-matrix simulations and phantom validation to improve the 2HG signal selectivity with respect to the neighboring resonances of γ-aminobutyric acid (GABA), glutamate (Glu), and glutamine (Gln). MRS data were acquired from 12 subjects with gliomas in vivo and analyzed with LCModel using calculated basis spectra. Metabolite levels were quantified using unsuppressed short echo time (TE) water as a reference.

Results

The PRESS TE was optimized as TE = 78 ms (TE₁ = 58 ms and TE₂ = 20 ms), at which the 2HG 2.25 ppm resonance appeared as a temporally max

SUBMITTER: Ganji SK 

PROVIDER: S-EPMC5026542 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature