Rasip1-Mediated Rho GTPase Signaling Regulates Blood Vessel Tubulogenesis via Nonmuscle Myosin II.

ABSTRACT:

Rationale

Vascular tubulogenesis is essential to cardiovascular development. Within initial vascular cords of endothelial cells, apical membranes are established and become cleared of cell-cell junctions, thereby allowing continuous central lumens to open. Rasip1 (Ras-interacting protein 1) is required for apical junction clearance, as well as for regulation of Rho GTPase (enzyme that hydrolyzes GTP) activity. However, it remains unknown how activities of different Rho GTPases are coordinated by Rasip1 to direct tubulogenesis.

Objective

The aim of this study is to determine the mechanisms downstream of Rasip1 that drive vascular tubulogenesis.

Methods and results

Using conditional mouse mutant models and pharmacological approaches, we dissect GTPase pathways downstrea

SUBMITTER: Barry DM 

PROVIDER: S-EPMC5026621 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature