Actinomyces spp. gene expression in root caries lesions.

ABSTRACT:

Background

The studies of the distribution of Actinomyces spp. on carious and non-carious root surfaces have not been able to confirm the association of these bacteria with root caries, although they were extensively implicated as a prime suspect in root caries.

Objective

The aim of this study was to observe the gene expression of Actinomyces spp. in the microbiota of root surfaces with and without caries.

Design

The oral biofilms from exposed sound root surface (SRS; n=10) and active root caries (RC; n=30) samples were collected. The total bacterial RNA was extracted, and the mRNA was isolated. Samples with low RNA concentration were pooled, yielding a final sample size of SRS=10 and RC=9. Complementary DNA (cDNA) libraries were prepared and sequenced on an Illumina(

SUBMITTER: Dame-Teixeira N 

PROVIDER: S-EPMC5027334 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature