The Mother Centriole Appendage Protein Cenexin Modulates Lumen Formation through Spindle Orientation.

ABSTRACT: Establishing apical-basal polarity is instrumental in the functional shaping of a solitary lumen within an acinus. By exploiting micropatterned slides, wound healing assays, and three-dimensional culture systems, we identified a mother centriole subdistal appendage protein, cenexin, as a critical player in symmetric lumen expansion through the control of microtubule organization. In this regard, cenexin was required for both centrosome positioning in interphase cells and proper spindle orientation during mitosis. In contrast, the essential mother centriole distal appendage protein CEP164 did not play a role in either process, demonstrating the specificity of subdistal appendages for these events. Importantly, upon closer examination we found that cenexin depletion decreased astral microtub

SUBMITTER: Hung HF 

PROVIDER: S-EPMC5027652 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature