Linkage between Fitness of Yeast Cells and Adenylate Kinase Catalysis.

ABSTRACT: Enzymes have evolved with highly specific values of their catalytic parameters kcat and KM. This poses fundamental biological questions about the selection pressures responsible for evolutionary tuning of these parameters. Here we are address these questions for the enzyme adenylate kinase (Adk) in eukaryotic yeast cells. A plasmid shuffling system was developed to allow quantification of relative fitness (calculated from growth rates) of yeast in response to perturbations of Adk activity introduced through mutations. Biophysical characterization verified that all variants studied were properly folded and that the mutations did not cause any substantial differences to thermal stability. We found that cytosolic Adk is essential for yeast viability in our strain background and that viability

SUBMITTER: Tukenmez H 

PROVIDER: S-EPMC5028032 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature